![]() ![]() The results showed that all samples contained bovine gelatin, and the absence of porcine gelatin was verified. Moreover, eight food products labeled as containing bovine gelatin and eight capsule shells were subjected to PCR examination. Although most of the DNA is degraded due to the severe processing steps of gelatin production, the minimum level of 0.1% w/w of both porcine and bovine gelatin was detected. The sensitivity of the method was tested on binary gelatin mixtures containing 0.1%, 1%, 10%, and 100% (w/w) of porcine gelatin within bovine gelatin and vice versa. ![]() The amplified expected PCR products of 212 and 271 bp were observed for porcine and bovine gelatin, respectively. ![]() After isolation of DNA from gelatin powders with known origin, conventional PCR using species-specific primers was carried out on the extracted DNA. In this study, a species-specific polymerase chain reaction (PCR) assay using conserved regions of mitochondrial DNA (cytochrome b gene) was performed to evaluate the halal authenticity of gelatin. To ensure that food products comply with halal regulations, development of valid and reliable analytical methods is very much required. Gelatin, mostly derived from bovine and porcine sources, has many applications in the food and pharmaceutical industries. Consumption of food products derived from porcine sources is strictly prohibited in Islam. ![]()
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